Figure 1.
Figure 1. Quantification of PRV-1 mRNA by Taqman RT-PCR. Total RNA (5 ng) from a PV patient (PV) and from a healthy control (HC) was subjected to quantitative RT-PCR for PRV-1 and GAPDH in an ABI PRISM 7700 Sequence Detection System. Fluorescence emitted during the PCR amplification (y-axis) is plotted against the cycle number (x-axis). For each reaction, the cycles of threshold (CT) are determined when a preset level of fluorescence is reached: one CT is obtained for PRV-1, another for GAPDH. The PRV-1/GAPDH ratio of a sample is determined by dividing the CTPRV-1by the CTGAPDH.

Quantification of PRV-1 mRNA by Taqman RT-PCR. Total RNA (5 ng) from a PV patient (PV) and from a healthy control (HC) was subjected to quantitative RT-PCR for PRV-1 and GAPDH in an ABI PRISM 7700 Sequence Detection System. Fluorescence emitted during the PCR amplification (y-axis) is plotted against the cycle number (x-axis). For each reaction, the cycles of threshold (CT) are determined when a preset level of fluorescence is reached: one CT is obtained for PRV-1, another for GAPDH. The PRV-1/GAPDH ratio of a sample is determined by dividing the CTPRV-1by the CTGAPDH.

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