Figure 4.
Detection of AAV-cFVIII-SQ transgene in dog liver. DNA was extracted 8 months after injection from the right and left liver lobes of the dog injected with 2.7 × 1013 vg/kg (Junior). Ten μg of DNA was digested with BamHI, Southern blotted, and hybridized with a 32P random-primer–labeled canine FVIII probe. Copy number standards were generated by spiking BamHI-digested naive canine Hem A liver DNA with BamHI-digested cF8104 plasmid DNA at ratios of 5, 1.25, 0.625, 0.313, and 0.16 copies/diploid genome.
