![Figure 2. Proliferation and IFNγ production by Vγ9Vδ2 cells from cancer patients before and after treatment with zoledronic acid. PBMCs were cultured at 37°C, in the presence of 5% CO2, at 106/mL in 96-well flat-bottomed plates (0.2 mL/well), with isopentenylpyrophosphate (IPP; Sigma Chemical, St Louis, MO; 100 μM/mL final concentration) and 20 U/mL final concentration human recombinant IL-2 (▪) or with IL-2 alone as a control (□). Proliferation (A) was measured 7 days later by adding 1 μCi/well (0.037 MBq) [3H]thymidine (Amersham, Arlington Heights, IL) during the last 6 hours of culture. Cells were then harvested and [3H]thymidine incorporation was measured with a liquid scintillation β-counter. Results are expressed as mean counts per minute (cpm) of triplicate wells ± standard deviation (SD). IFNγ levels in the 48-hour culture supernatants (B) were assessed by 2 monoclonal antibodies (mAbs) sandwich enzyme-linked immunosorbent assay (ELISA) assay following the manufacturer's recommendations (R&D Systems, Minneapolis, MN).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/6/10.1182_blood-2003-05-1655/6/h81835050002.jpeg?Expires=1735515325&Signature=HyWN~SBoRTaAG1~eJgsZlcqTTLWsnk~SZHgW5PwFSOBm3DcS01KUNilZSVqRYnWPVeeICzN3jbIl7-U-3XVWD321FjvB1A1D1FZA2keONoJe8arj61cORcLiwFzOhAdqTf1eUAM~EgqKb6x7gDfyu5n8f~r5Z8cbTj8o2Z-OmqqWM7DhsApw4oRNLBZeGOCEP0sRjnFBFJLU8YOeeg7uHDof3vG8m2mU2daFexp2yBHlx7Ily~X875TpJEF-CrD~n3hfp8pB72NCS8~O1vO2mRuEJ660JAmcvHZrt~pCIpc5m39Rn~OzMxWNxEIXiH-VplXKFA0mmQB10TLL285ABg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Proliferation and IFNγ production by Vγ9Vδ2 cells from cancer patients before and after treatment with zoledronic acid. PBMCs were cultured at 37°C, in the presence of 5% CO2, at 106/mL in 96-well flat-bottomed plates (0.2 mL/well), with isopentenylpyrophosphate (IPP; Sigma Chemical, St Louis, MO; 100 μM/mL final concentration) and 20 U/mL final concentration human recombinant IL-2 (▪) or with IL-2 alone as a control (□). Proliferation (A) was measured 7 days later by adding 1 μCi/well (0.037 MBq) [3H]thymidine (Amersham, Arlington Heights, IL) during the last 6 hours of culture. Cells were then harvested and [3H]thymidine incorporation was measured with a liquid scintillation β-counter. Results are expressed as mean counts per minute (cpm) of triplicate wells ± standard deviation (SD). IFNγ levels in the 48-hour culture supernatants (B) were assessed by 2 monoclonal antibodies (mAbs) sandwich enzyme-linked immunosorbent assay (ELISA) assay following the manufacturer's recommendations (R&D Systems, Minneapolis, MN).
