Figure 1.
Figure 1. GFP Ad vector induced HSP70 protein expression in HUVECs. (A) Cells were treated with culture medium (control), AdNull 200 MOI, AdLuciferase 200 MOI, AdGFP 200 MOI, and Adβ-galactosidase (Adβ-gal) 200 MOI, and cultured in growth factor–free medium for 48 hours. Cellular extracts were prepared from HUVECs, and HSPs were detected by Western blot analysis using antibodies specific for HSP70 and β-actin. (B) HUVECs were infected for 48 hours with control or AdGFP at the indicated concentrations ranging from 50 to 400 MOI. Immunoblots were probed with antibodies specific to HSP70, HSC70, HSP90, HO-1, and β-actin. (C) HUVECs were infected with control, AdNull (200 MOI), or AdGFP (200 MOI) for the indicated time periods. Immunoblots were probed with antibodies specific for HSP70, HSC70, GFP, and β-actin. The results shown represent one sample experiment from a total of 3 independent experiments.

GFP Ad vector induced HSP70 protein expression in HUVECs. (A) Cells were treated with culture medium (control), AdNull 200 MOI, AdLuciferase 200 MOI, AdGFP 200 MOI, and Adβ-galactosidase (Adβ-gal) 200 MOI, and cultured in growth factor–free medium for 48 hours. Cellular extracts were prepared from HUVECs, and HSPs were detected by Western blot analysis using antibodies specific for HSP70 and β-actin. (B) HUVECs were infected for 48 hours with control or AdGFP at the indicated concentrations ranging from 50 to 400 MOI. Immunoblots were probed with antibodies specific to HSP70, HSC70, HSP90, HO-1, and β-actin. (C) HUVECs were infected with control, AdNull (200 MOI), or AdGFP (200 MOI) for the indicated time periods. Immunoblots were probed with antibodies specific for HSP70, HSC70, GFP, and β-actin. The results shown represent one sample experiment from a total of 3 independent experiments.

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