GFP-induced HSP70 protein expression specific in ECs. Non-ECs: HuSMCs (A) orA549 cell line (B) were infected with AdGFP (50-500 MOI) for 48 hours. ECs: HDMECs (C), BMECs (D), or HMVECs (E) were infected with AdGFP or AdNull at the indicated concentrations ranging from 50 to 200 MOI for 48 hours. Cell lysates were then assayed for HSP70, GFP, and β-actin by Western blotting.