AdGFP-induced transcriptional activation of HSP70 promoter and RNA expression. (A) Northern analysis of HSP70 gene expression in ECs. Total RNA (10 μg/lane) was isolated from uninfected control cells and cells infected with AdNull (200 MOI) or AdGFP (50-200 MOI) for 48 hours. The blot was hybridized to a 32P-labeled human HSP70b cDNA probe and control 32P-labeled GAPDH probes to normalize RNA loading. (B) AdGFP activates HSP70 promoter activity. HUVECs were transfected with 2 μg HSP70 reporter plasmid containing the β-galactosidase for 12 hours, and then treated for 48 hours under the following conditions: control, AdNull 200 MOI, or AdGFP 200 MOI. β-Galactosidase reporter activities were measured in cellular extract. The columns are the means, and the bars are the SD; *P < .001 compared with control. The results are representative of 3 separate experiments.
