Blood loss. The blood loss is expressed as the amount of hemoglobin bled from the tail into the container with saline as nmol hemoglobin per mL saline. A indicates control; B, hemophilia control; C, hemophilia/rFVIIa (1 mg/kg); D, hemophilia/rFVIIa (3 mg/kg); E, hemophilia/rFVIIa (6 mg/kg); F, hemophilia/rFVIIa (10 mg/kg); G, hemophilia/FVIIaIIa (1 mg/kg); H, hemophilia/FVIIaIIa (3 mg/kg); I, hemophilia/K337A-FVIIaIIa (1 mg/kg); J, hemophilia/K337A-FVIIaIIa (3 mg/kg); K, hemophilia/M298Q-FVIIa (1 mg/kg); and L, hemophilia/M298Q-FVIIa (3 mg/kg). Data are presented as bars plus SD. Statistical significance was tested, using one-way analysis of variance (ANOVA). In cases of P < .05, pair-wise comparisons were made at the same level and corrected for multiple comparisons (Dunnett) testing all groups to the hemophilia control (B). The blood loss was increased significantly in the hemophilia control (B) compared with the normal control (A; ***P < .001). The blood loss was significantly decreased after administration of 3 (P < .001), 6 (P < .001), and 10 (P < .001) mg/kg rFVIIa, 1 (**P < .01) and 3 (P < .001) mg/kg FVIIaIIa, 1 (*P < .05) and 3 (P < .01) mg/kg K337A-FVIIaIIa, and 1 (P < .05) and 3 (P < .001) mg/kg M298Q-FVIIa compared with the hemophilia control.
