Figure 3.
Elevated serum IFN-γ levels in association with RLI in mixed allogeneic chimeras. BALB/c recipient mice were conditioned with anti-CD4– and anti-CD8–depleting mAbs on day –5, CTX 200 mg/kg intraperitoneally on day –1, and 7 Gy thymic irradiation on day 0 prior to transplantation of 20 × 106 B10.BR BMCs. The indicated group received 3 × 107 recipient spleen cells (RLI) from BALB/c mice on day +49 after BMT. Mean (± SEM) of serum IFN-γ levels measured 7 days after RLI are shown. Mice received conditioning without BMT or RLI (no BMT; n = 5), BMT without RLI (no RLI chimeras; n = 9), or received BMT and RLI and lost chimerism by 1 week after RLI (RLI chimeras; n = 7).
