FACS analysis and colony assay of the normal and anemic spleen ofG1-HRD-GFPtransgenic mouse. (A) Spleen mononucleated cells (MNCs) were obtained from G1-HRD-GFP transgenic mice before (PHZ-, left) and after (PHZ+, right) injection of phenylhydrazine (PHZ). The MNC, Lin^- fraction, and Lin^-/c-Kit⁺ fraction were analyzed by FACS (top, middle, and bottom panels, respectively). The percentage in each quadrangle is shown. The dotted lines show the mean intensities of CD71 expression in CD71⁺/GFP⁺ fractions (bottom panels). (B) Results of colony assay in the normal and anemic spleens. Two thousand cells from the indicated fractions were analyzed. Fractions i and ii are shown by open boxes in A. (C) The cell number of the erythroid fractions of the spleen were increased by PHZ injection. Fold increases between the PHZ-induced anemic spleens, and normal spleens are indicated in this graph. The results are shown with standard deviations.