HA-2-specific cytotoxic response of the various HA-2 TCR-modified T-cell lines. HA-2 TCR-modified T cells were tested in a cytotoxicity assay against HLA-A2-positive HA-2-positive target cells (EBV-RZ; ▦) and HLA-A2-positive HA-2-negative target cells (EBV-Z; □) at an effector-target (E/T) ratio of 10:1. As negative control, T cells transduced with retroviral vectors encoding for marker genes only were used. The cytotoxicity assay was performed for 4 hours.