HA-2-specific cytolytic reactivity of chimeric TCRs. T-cell clones transduced with chimeric TCR complexes, consisting of the TCR α chain derived from one HA-2-specific T-cell clone and the TCR β chain from another HA-2-specific T-cell clone, were tested in a cytotoxicity assay against HLA-A2-positive HA-2-positive target cells (EBV-RZ; ▪) and HLA-A2-positive HA-2-negative target cells (EBV-Z; □) at an E/T ratio of 10:1. For every chimeric TCR combination, 24 TCR-transferred T-cell clones were tested. The composition of the chimeric TCR combinations are indicated above each panel. The cytotoxicity assay was performed for 4 hours.