Figure 7.
Effect of anti-C5 and anti-C5a onN meningitidis-induced complement activation. Generation of C3bc (left panel) and TCC (right panel) in lepirudin-anticoagulated human whole blood after incubation for 1 hour at 37°C with 1 × 108 heat-inactivated N meningitidis H44/76 in the presence of anti-C5 (clone 137-76, 50 μg/mL), anti-C5a (clone 137-26, 50 μg/mL), or isotype control antibody (50 μg/mL). Formation of TCC was completely blocked by anti-C5 but unaffected by anti-C5a. Median ± IQRs of 4 separate experiments are presented. *P < .05 compared with isotype control.
