Adoptive transfer ofAspergillus-pulsed DCs protects mice with bone marrow transplants from aspergillosis. Lethally irradiated C3H/HeJ mice each received a transplant with at least 2 × 10⁶ T cell-depleted allogeneic bone marrow cells from BALB/c mice 2 weeks before the intratracheal infection with 2 × 10⁸Aspergillus conidia. One and 7 days after transplantation, mice received 5 × 10⁵ pulsed DCs subcutaneously. Treatment with the anti-Gr-1 antibody (1 mg intraperitoneally) was done the day of the infection. (A) MST (days) and CFUs of infected mice on adoptive transfer of DCs pulsed with different stimuli or unpulsed (none). In parentheses are the number of dead animals over total injected. (B) Cell recovery into the lungs of infected mice, as determined by FACS analysis. Staining for CD69 and CD25 was done on gated CD4⁺ cells. (C) Frequency of cytokine-producing CD4⁺ lung T lymphocytes as determined by ELISPOT assay. CFU, FACS analysis, and ELISPOT assay were performed at 6 days after the infection. ^*P < .05 (mice receiving pulsed DCs versus mice receiving unpulsed DCs). ▪, unpulsed DCs; ▧, conidia-pulsed DCs; ▦, conidial RNA-transfected DCs.