Effect of BSO on intracellular GSH levels. (A) T cells, NK cells, monocytes, immature DCs, and mature DCs (0.5 × 106/mL) were treated with BSO (5 mM) for 24 hours. Untreated cells were used as controls. Cells were then washed, and GSH levels were measured by HPLC. Results are expressed as nM GSH/mg protein and represent the mean ± SD from 3 separate experiments. *P < .05; **P < .01. (B) Cells were treated as in panel A, GSH-OEt was added at a final concentration of 0.4 mM for 5 hours, and GSH levels were measured by HPLC. Untreated cells were used as controls. Results are expressed as a percentage of control values and represent the mean ± SD from 3 separate experiments. (C) T cells, NK cells, monocytes, immature DCs, and mature DCs (0.5 × 106/mL) were treated with BSO (0.5 mM) for 24 hours. GSH-OEt was added at a final concentration of 5 mM for 5 hours. Cells were then washed, and GSH levels were determined. Untreated cells were used as controls. Results are expressed as the percentage of control values and represent the mean ± SD from 3 separate experiments. *P < .05; **P < .01.
