CIB sequence and areas of localization are highly conserved among species. (A) Amino acid sequences of CIB in humans, rats, mice, chicken, and fish were analyzed by multiple sequence alignment. Those amino acids that differ from the human sequence are highlighted in red, and EF-hand motifs are boxed; asterisks indicate aminoacid identity. (B) Multiple sequence alignment dendrogram of CIB showing the evolutionary relationship between the orthologues. (C) Immunofluorescence images of CIB localization in a variety of cell types. Platelets were allowed to attach on fibrinogen-coated glass cover slides for 45 minutes, then fixed and stained with UN2 for CIB (red) and FITC-conjugated phalloidin for F-actin (green). Scale bar, 5 μm. HUVECs, CHO, and NIH3T3 cells were allowed to spread on fibronectin-coated cover glass for 45 minutes, then fixed and stained as above. Scale bar, 20 μm.