CIB induces cell migration through integrin α₅β₁ (A) Mock- and CIB-overexpressed cells were allowed to migrate for 5 hours across transwell inserts coated on the underside with fibronectin. Unmigrated cells were removed, and migrated cells were fixed and stained. Original magnification, × 200. (B) Mock- and CIB-overexpressed cells were incubated with increasing concentrations of function-blocking anti-α₅β₁ for 20 minutes and were then allowed to migrate across fibronectin-coated inserts in the presence of function-blocking antibody for 5 hours. Error bars indicate mean ± SEM of at least 3 independent experiments.