Comparison of Ad5 and Ad5/F35 infectivity in EBV-LCL cells. EBV-LCL cells were cocultured for 18 hours with 1500 pfu per cell of Ad5-EGFP or 200 pfu per cell of Ad5/F35-EGFP, and then washed. At 48 hours after infection, the cells were analyzed for EGFP or surface molecule expression by flow cytometry. (A) The results are expressed as the percentage of EGFP-expressing cells. (B) EBV-LCLs transduced by Ad5/F35-Jagged-1 showed the same profile of surface expression as control, MHC class I and II, and costimulatory ligands (ie, HLA-A, -B, -C; HLA-DR; CD40; CD80; and CD86). For each assay, 1 representative experiment of 4 is shown.