Jagged-1 expression in Ad5/F35-Jagged-1-transduced EBV-LCL cells. Transduction efficiency of EBV-LCLs using Ad5/F35-Jagged-1 was monitored after 48 hours by Western blotting and RQ-PCR. (A) Jagged-1 protein (≈180 kDa) was detected in EBV-LCLs 48 hours after transduction by Ad5/F35-Jagged-1 vector. Human bone marrow stromal cells were used as the positive control for Jagged-1 protein expression. (B) RQ-PCR assay showed a mean 62-fold higher quantity of Jagged-1 mRNA in EBV-LCLs after transduction by Ad5/F35-Jagged-1 vector (gray bar), compared with control-transduced EBV-LCLs (black bar) (mean ± SD of 3 experiments). (C) To confirm the functionality of Jagged-1 expressed on transduced LCLs, T-cell populations were analyzed for HES-1 and Deltex mRNA content after 12 hours of activation in an MLC (mean ±SD of 3 experiments).