Tissue distribution of HD vector genomes after gene transfer. Total gDNA was isolated from different organs from both dogs, G11 and G21. The biodistribution study was performed 5.5 months after injection for dog G11 and 2 months after injection for G21. For regular PCR analyses, 500 ng genomic DNA from various tissues was used. The genomic DNA liver samples from both dogs were analyzed by PCR (A) and quantitative Southern Blot analyses (B). Detection of vector genomes in different organs from dog G11 (C) and G21 (D). Liver 1, caudate; liver 2, right medial; liver 3, quadrate; liver 4, left; liver 5, right lateral; muscle, intercostal muscle; gut, duodenum; and brain, medulla.