American Society of Hematology

Figure 1.

$Figure 1. Induction of apoptosis in K562 cells by SAHA or SB and bortezomib. (A) K562 cells were exposed for 48 hours to 1.5 μM SAHA in conjunction with the indicated concentration of bortezomib (btzmb), after which apoptosis was monitored by morphologic analysis of Wright Giemsa-stained specimens as described in “Materials and methods.” (B) Cells were exposed for 48 hours to 4.5 nM bortezomib in conjunction with the designated concentration of SAHA, after which apoptosis was assessed as above. (C) K562 cells were exposed to varying concentrations of SAHA and bortezomib at a fixed ratio (333:1) for 48 hours after which combination index (CI) values for apoptosis were determined in relation to the fraction affected (FA) using median dose effect analysis. CI values less than 1.0 correspond to a synergistic interaction. (D) K562 cells were exposed to 1.5 μM SAHA ± 4.5 nM bortezomib for the indicated intervals, after which the percentage of apoptotic cells was assessed as described in “Materials and methods.” In each case, values represent the means ± SD for 3 separate experiments.$

Induction of apoptosis in K562 cells by SAHA or SB and bortezomib. (A) K562 cells were exposed for 48 hours to 1.5 μM SAHA in conjunction with the indicated concentration of bortezomib (btzmb), after which apoptosis was monitored by morphologic analysis of Wright Giemsa-stained specimens as described in “Materials and methods.” (B) Cells were exposed for 48 hours to 4.5 nM bortezomib in conjunction with the designated concentration of SAHA, after which apoptosis was assessed as above. (C) K562 cells were exposed to varying concentrations of SAHA and bortezomib at a fixed ratio (333:1) for 48 hours after which combination index (CI) values for apoptosis were determined in relation to the fraction affected (FA) using median dose effect analysis. CI values less than 1.0 correspond to a synergistic interaction. (D) K562 cells were exposed to 1.5 μM SAHA ± 4.5 nM bortezomib for the indicated intervals, after which the percentage of apoptotic cells was assessed as described in “Materials and methods.” In each case, values represent the means ± SD for 3 separate experiments.

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