American Society of Hematology

Figure 3.

$Figure 3. Induction of mitochondrial injury and caspase activation in K562 cells by SAHA or SB and bortezomib. K562 cells were exposed to 1.5 μM SAHA ± 4.5 nM bortezomib for 48 hours, after which Western analysis was used to assess release of AIF, Smac/DIABLO, and cytochrome cinto S-100 cytosolic fractions, and total cellular extracts were monitored for expression of cleaved caspase-9, caspase-3, caspase-8, PARP, and Bcr/Abl. Each lane contained 25 μg protein; blots were stripped and reprobed for tubulin to ensure equivalent loading and transfer. An additional 2 studies yielded equivalent results.$

Induction of mitochondrial injury and caspase activation in K562 cells by SAHA or SB and bortezomib. K562 cells were exposed to 1.5 μM SAHA ± 4.5 nM bortezomib for 48 hours, after which Western analysis was used to assess release of AIF, Smac/DIABLO, and cytochrome cinto S-100 cytosolic fractions, and total cellular extracts were monitored for expression of cleaved caspase-9, caspase-3, caspase-8, PARP, and Bcr/Abl. Each lane contained 25 μg protein; blots were stripped and reprobed for tubulin to ensure equivalent loading and transfer. An additional 2 studies yielded equivalent results.

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