Morphology of flow-sorted rhesus DC subsets. Rhesus pre-DC subsets were stained with Giemsa (A-D) or examined using SEM (E-H) before and after culture with DC maturation-inducing factors GM-CSF and CD40L. (A) Freshly sorted CD11c⁺ pre-DCs showing a high nucleus-cytoplasm ratio, reniform or multilobulated nuclei, and few prominent dendrites. (B) Freshly sorted CD123⁺⁺ pre-DCs with a typical prominent Golgi region and lateralized reniform nucleus. (C) After culture with rhGM-CSF and rhCD40L for 24 hours, CD11c⁺ pre-DCs showed abundant and well-developed dendrites. (D) After culture with rhIL-3 and CD40L for 3 days, CD123⁺⁺ pre-DCs also acquired striking dendritic morphology. (E-H) SEM images of cells corresponding to populations in panels A to D Original magnifications: × 400 (A-D); × 7500 (E); × 9000 (F); × 5000 (G); and × 3500 (H).