Localization of rhesus DC subsets within normal and Flt3L-treated rhesus monkey lymph nodes. (A) Normal lymph node: germinal center CD11c⁺ myeloid DCs (green) are visible as CD11c⁺ cells with dendritic morphology within follicular (F) and parafollicular (P) regions (original magnification × 200). (B) p55 (Fascin)-stained DC (green) colocalizing with CD123-biotin (red) to indicate preplasmacytoid DCs within normal lymph node (orange/yellow; inset magnification × 600). (C) Cells expressing CD123 in Flt3L-mobilized rhesus monkeys tended to occur in aggregates (red), whereas CD11c⁺ cells (green) were visible as isolated cells (original magnification × 100). (D) Ki-67 staining of lymph node from an Flt3L-treated rhesus monkey, showing CD123⁺ cells (red) in clumps distinct from Ki-67-positive cells (green), predominantly within the follicle (F) (original magnification × 200).