TRMA cells without thiamine exhibit decreased RNA ribose synthesis via transketolase. The fraction of RNA ribose derived from the oxidative (G6PD) and nonoxidative (TK) pathways is shown on the x-axis. The figure shows the significant rearrangement of oxidative/nonoxidative ribose synthesis pathways in TRMA cells, primarily shifting toward oxidative synthesis, while wild-type cells maintain metabolic activities and identical substrate flow in the pentose cycle after thiamine depletion. (Mean ± SD; n = 9) ^*P < .001.