Comparable surface expression levels of ICAM-1 wild type and ICAM-1 mutants on bEndI1/2.1 and bEndI1.1. The surface expression levels of ICAM-1 wild type and ICAM-1 mutants transduced into bEndI1/2.1 (A) and bEndI1.1 (B) is shown as determined by FACS analysis. Endothelial cells are scatter gated on live cells; overlays show specific staining for ICAM-1 (thick lines) compared with control antibody staining (thin lines). In panel A, the surface expression level for ICAM-2 transduced into bEndI1/2.1 is included. In panel B, the surface expression level for the ICAM-1/ICAM-2 chimera I1EI2C is included. ^*This staining was performed separately.