Acute DOX treatment induces alterations in iron metabolism that are exacerbated in Hfe-deficient mice. Iron concentration in the heart (A), liver (B), spleen (C), and pancreas (D) of saline-treated (white symbols) and DOX-treated, day 4, (black symbols), wild-type (○, •) and Hfe^-/- (□, ▪) mice. Horizontal bars represent means. Differences between strains and between treatments were determined by Student t test.