Iron storage in hepatic cells and heart after acute DOX treatment in wild-type and Hfe-deficient mice (Prussian blue staining). Light micrographs of liver (A-D) and heart (E-F) sections from saline-treated (A-B and E-F) or DOX-treated, day 4, (C-D and G-H) wild-type and Hfe^-/- mice. In the livers of Hfe^-/- mice, parenchymal cells with increased iron content after DOX treatment are visible around the portal spaces. In DOX-treated wild-type mice, iron could be detected only in 2 of 8 mice at day 4 (C) and 2 of 7 mice at day 12 and was found in Kupffer cells (arrows, C-D). In the heart, iron was detected in cardiomyocytes of DOX-treated Hfe^-/- mice (H). Original magnification, × 400.