Effect of Hfe deficiency on DOX-induced increase in serum creatine kinase (CK), aspartate aminotransferase (AST), and alanine aminotransferase (ALT). Serum CK (A), AST (B), and ALT (C) were measured in saline-treated (white symbols) and DOX-treated, day 4, (black symbols) wild-type (○, •) and Hfe-/- (□, ▪) mice. Horizontal bars represent means. Differences between strains and between treatments were determined by Student t test.