Figure 2.
Fluorescence resonance energy transfer showing close physical proximity of FcγRIIa with platelet glycoprotein receptors, PECAM-1, and GPIbα Fluorescence resonance energy transfer efficiency values between platelet membrane epitopes using monoclonal antibody F(ab′)2 fragments to glycoprotein Ibα (VM16d), PECAM-1 (PEC-1.1, PEC-1.2), and FcγRIIa (IV.3). Energy transfer was measured between FITC-labeled (donor excitation) and Alexa-546-labeled (acceptor emission) epitopes using a luminescence spectrometer. Results are representative of 4 experiments.
