Figure 3.
Coimmunoprecipitation of PECAM-1 and FcγRIIa from digitonin-lysed platelets. (A) Washed platelets were treated with and without chemical cross-linking prior to digitonin lysis and immunoprecipitation using antibodies against the polypeptides: normal mouse IgG1, FcγRIIa (IV.3), PECAM-1 (PEC-1.1), and CD151 (11B1). Immunoblot shown reveals presence of PECAM-1 antigen. (B) Same as panel A. The only exception is that the immunoblot is probed for the presence of CD151 under nonreduced conditions. NM indicates normal mouse IgG; NXL, nonchemically crosslinked; and XL, chemically crosslinked.
