Figure 3.
Figure 3. A soluble factor secreted by MSCs on activation mediates the immunosuppressive effect. The proliferation of 105 BALB/c splenocytes triggered by 105 mitomycin-treated allogeneic C3H or DBA1 splenocytes (allo), in a 4-day MLR, was assigned a 100% value ± SD. The proliferative response was measured in the presence of 105 C3 MSCs, either in direct contact with splenocytes (+ MSC) or separated from splenocytes by a 1-μm transwell chamber (MSC + transwell). Supernatants from cultured C3 MSCs (MSC supant) or conditioned supernatants from C3 MSCs previously “activated” by a 4-day culture with allogeneic splenocytes (conditioned supant) were tested.

A soluble factor secreted by MSCs on activation mediates the immunosuppressive effect. The proliferation of 105 BALB/c splenocytes triggered by 105 mitomycin-treated allogeneic C3H or DBA1 splenocytes (allo), in a 4-day MLR, was assigned a 100% value ± SD. The proliferative response was measured in the presence of 105 C3 MSCs, either in direct contact with splenocytes (+ MSC) or separated from splenocytes by a 1-μm transwell chamber (MSC + transwell). Supernatants from cultured C3 MSCs (MSC supant) or conditioned supernatants from C3 MSCs previously “activated” by a 4-day culture with allogeneic splenocytes (conditioned supant) were tested.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal