Figure 1.
![Figure 1. Dissociation of radiolabeled WT- and ΔK-scuPA from soluble uPAR. suPAR-coated wells were incubated with 125I-WT-scuPA (5 [▾], 10 [▴], and 20 nM [▪]) or 125I-ΔK-scuPA (5 [▿], 10 [▵], and 20 nM [□]) for 2 hours at 22°C. Wells were washed 4 times, and the released radioactive ligand was counted at the indicated times. The lines represent the best fit to the single exponential decay model as determined using GraphPad software.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/10/10.1182_blood-2003-03-0949/6/h82235250001.jpeg?Expires=1735561448&Signature=Ba6fQ7LOnnZhW5PiTLCFvy8Y94BdHNWlxuj3xsLEEsSI57RZAXoJbgBrimcOrxvc2~vDfWc1eLgs~y-zRFOu86u~LkU3UEJ~G7arBsx3g7Hm~QKYpj4CNvzNlHB3CrT2aKnFjAdhpH-lCMdEQlcGgBak00mGsBNO0YZMwLnNAPQrBeW07D7zPgHER4aRT2hu0czvJWzSMfBIKfFLrERZ8fcqzoFbThfTx2aMrFfu0TE~m3TzZsHl9DcaxTyaVD~wsfyiuHANy5Wtea-xq70QwgvCpCMzqLNWsltcppGZd2WEvKbvvHnU4jTyUQK8dkYZEdJF~HNhjzwevPfsneiVjQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Dissociation of radiolabeled WT- and ΔK-scuPA from soluble uPAR. suPAR-coated wells were incubated with 125I-WT-scuPA (5 [▾], 10 [▴], and 20 nM [▪]) or 125I-ΔK-scuPA (5 [▿], 10 [▵], and 20 nM [□]) for 2 hours at 22°C. Wells were washed 4 times, and the released radioactive ligand was counted at the indicated times. The lines represent the best fit to the single exponential decay model as determined using GraphPad software.
