Expression pattern of genes that distinguish MM1 from MM4 under different treatments. (A) Twenty-four of 30 genes that distinguished MM1 from MM4 classes reported by Zhan et al10 were found to be present in at least one response group. Seven genes are depicted as a ratio of their average difference over the average difference of NIL controls. A/S indicates stroma-stimulated samples; IL-6, IL-6-treated samples, N-ras, mutant N-ras transfectants. (B) RT-PCR confirms the microarray data. Total RNA was isolated using the RNA Easy kit (Qiagen); cDNA was made and amplified by PCR using specific primers for mip-1α, EZH2, and GAPDH as loading control. A indicates ANBL6 IL-6-starved; N-Ras, ANBL-6 stably transfected with activated N-ras; A/S, ANBL-6 stimulated with stroma; IL-6, ANBL-6 stimulated with 10 ng recombinant IL-6; S, stroma.
