Inhibition of TNFα by MyD-1 is regulated by a wortmannin-sensitive signaling cascade, but IL-6 secretion is not. Human PBMCs were cultured in the presence or absence of 100 ng/mL LPS, 10 μg/mL ILA24 mouse anti-human MyD-1, and 1 nM wortmannin for 24 hours, after which supernatants were analyzed for TNFα or IL-6 production. The results shown are the mean production of (A) TNFα or (B) IL-6 secretion in the presence of 1 nM wortmannin. The data are generated from triplicate samples and are shown as the mean concentration ± 1 SD. In the absence of 1 nM wortmannin, the levels of TNFα produced by anti-MyD-1 plus LPS compared with LPS are significantly reduced, *P < .001, but in the presence of 1 nM wortmannin there is no significant difference between any groups. These data are representative of 4 experiments.