The anti-MyD-1 mAb ILA24 does not block binding of a MyD-1Ig fusion protein to CD47 expressed on lymphocytes. Bovine PBMCs were incubated with MyD-1Ig (5 μg/mL) that had been preincubated with either the anti-MyD-1 mAb ILA24 (open histogram, A), the anti-MyD-1 mAb CC149 (open histogram, B), or the relevant isotype-matched control mAbs (filled histograms). In addition, PBMCs were preincubated with the anti-CD47 mAb MEM-122 (open histogram, C) prior to addition of the MyD-1 fusion protein. All antibodies were used at a concentration of 10 μg/mL. MyD-1 binding was detected by labeling with FITC-conjugated goat anti-human IgG and FACS analysis of lymphocytes gated on the basis of forward and side scatter.