The effect ofHexdeletion on in vitro vascular sprout formation in differentiating EBs. (A) The 4 classes of vascular sprout development in EBs, as described in “Materials and methods.” Original magnification, × 10. (B) The percent of EBs in each class by Hex genotype. There were fewer CD31⁺ mature vascular tubules in the Hex^-/- EBs. Assays were preformed 3 times in triplicate. Error bars represent SEM.