Analysis of the ability ofHex^-/-ES cells to contribute hematopoietic tissues in chimeric mice. The ratio of Hex^-/- DNA to Hex^+/+ DNA in specific organs or flow cytometrically sorted individual hematopoietic cell types was obtained using normalized multiplex PCR. The expected ratio would be 1.0 if both Hex^-/- and Hex^+/+ cells contributed without bias to tissue development. There were only rare contributions from Hex^-/- ES cells to any hematopoietic cell type. Aorta will contain smooth muscle, which does not require Hex. Each ratio is the averaged value from 4 distinct mice analyzed individually. Error bars represent SEM. Inset shows genomic PCR of Hex chimeric mice.