Figure 5.
![Figure 5. Functional properties of purified subsets after IL-15-stimulation. The purified subsets were labeled with CFSE and stimulated with IL-15 (10 ng/mL). After 7 days the cells were restimulated with PMA/ionomycine and brefeldin A and stained intracellular for indicated cytokines followed by FACS analysis (gamma-1 [g-1] used as control mAbs). (A) CD8+ T cells purified from UCB. (B) Peripheral naive CD8+ T cells. (C) Cytotoxic CD8+ T cells (CD27-CD45RAbright). (D) Noncytotoxic CD8+ T cells (CD27+CD45RAdull/neg). Data are one representative experiment of 4 performed.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/7/10.1182_blood-2003-01-0183/6/h81934985005.jpeg?Expires=1735850139&Signature=fevZhRKL4gNcrZC7qliTl0V2p3QCpQLrYFU5VZQqMowCDG4FJB-ipRD0sSKNJygBTKae5J5n7RqwHL9aQh86EwLbSUH6jTONfl3AlvQVofC9c18hzEnEd-yna0VmZs8nf435gCsmtSu~gYwNbELfwLo1t5KvwGE3Vf7Hf9-lZApvvqAqZPS0JKAVlBTyLSfuYeaNqG1r3dYHeFu7l4aQ9Xnu2wfH6rGv1XZdJ1MmJhmuocBMAl90Fh6XLxnzU~E2~AibyuoiXPSugKKGOkeH28EVHdZWVqlmyvlWoemFUxYe1WtIk9tw0x9DrSbMKYUwWBMUKKsQ~ptGbNRE8dAf2w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Functional properties of purified subsets after IL-15-stimulation. The purified subsets were labeled with CFSE and stimulated with IL-15 (10 ng/mL). After 7 days the cells were restimulated with PMA/ionomycine and brefeldin A and stained intracellular for indicated cytokines followed by FACS analysis (gamma-1 [g-1] used as control mAbs). (A) CD8+ T cells purified from UCB. (B) Peripheral naive CD8+ T cells. (C) Cytotoxic CD8+ T cells (CD27-CD45RAbright). (D) Noncytotoxic CD8+ T cells (CD27+CD45RAdull/neg). Data are one representative experiment of 4 performed.
