Figure 2.
Expression of the SSH6 gene in a variety of human tissues and cell lines. Northern blot analysis of a commercially available human multiple-tissue expression array (MTE; Clontech). Hybridization with a probe corresponding to (A) exon 2-3-4 and (B) exon 3. Samples 1 to 61 correspond to poly A+ RNA from human tissues (1, whole brain; 2, cerebral cortex; 3, frontal lobe; 4, parietal lobe; 5, occipital lobe; 6, temporal lobe; 7, paracentral gyrus of cerebral cortex; 8, pons; 9, left cerebellum; 10, right cerebellum; 11, corpus callosum; 12, amygdala; 13, caudate nucleus; 14, hippocampus; 15, medulla oblongata; 16, putamen; 17, substantia nigra, 18, accumbens nucleus; 19, thalamus; 20, pituitary gland; 21, spinal cord; 22, heart; 23, aorta; 24, left atrium; 25, right atrium; 26, left ventricle; 27, right ventricle; 28, interventricular septum; 29, apex of the heart; 30, esophagus; 31, stomach; 32, duodenum; 33, jejunum; 34, ileum; 35 ilocecum; 36, appendix; 37, ascending colon; 38 transverse colon; 39, descending colon; 40, rectum; 41 kidney; 42, skeletal muscle; 43, spleen; 44, thymus; 45, peripheral blood leukocyte; 46, lymph node; 47, bone morrow; 48, trachea; 49, lung; 50, placenta; 51, bladder; 52, uterus; 53, prostate; 54, testis; 55, ovary; 56, liver; 57 pancreas; 58, adrenal gland; 59, thyroid gland; 60, salivary gland; 61, mammary gland); from human cell lines (62, leukemia HL-60; 63, HeLa S3; 64, leukemia, K-562; 65, leukemia MOLT-4; 66, Burkitt lymphoma Raji; 67, Burkitt lymphoma Daudi; 68, colorectal adenocarcinoma, A549); from fetal tissues (69, fetal brain; 70, fetal heart; 71, fetal kidney; 72, fetal liver; 73, fetal spleen; 74, fetal thymus; 75, fetal lung); and from controls (76, yeast total RNA; 77, yeast tRNA; 78, E coli rRNA; 79, E coli DNA; 80, Poly r(A); 81, human C0t-1 DNA; 82, 100 ng human DNA; 83, 500 ng human DNA). Panels at the right show the hybridization signal of the exon 3 probe with vascular SMC–derived RNA and exon 3 containing plasmid DNA, respectively.