FISH analysis with the patients using various centromeric alphoid probes. Interphase FISH with a D1Z5 probe, CEP1, in normal (A) and abnormal (B) cells. Two-color FISH with pE25.a (green) and D7Z1^6mer (red) with tumor cells, showing almost completely overlapped signals (arrows) on metaphase (C) and interphase (D) nuclei. Original magnification, × 1000. (E) Fiber FISH analysis of der(1;7)(q10;p10) using a D1Z7 probe, CEP1/5 (red), and a D7Z1 probe, CEP7 (green), visualizing direct connection of both alphoids on the same DNA fiber.