Expression of protease-activated receptors in human dendritic cells. (A) RT-PCR analysis shows PAR1, PAR2, and PAR3 mRNA expression in dendritic cells differentiated from monocytes for 6 days in the presence of 1000 U/mL GM-CSF and 25 ng/mL IL-4. GAPDH was used for normalization. (B) Flow cytometric analysis of PAR1, PAR2, and PAR3 in human dendritic cells was performed as described in Figure 1. CD14 and CD1a antigens were used as differentiation markers. Nonshaded peaks represent cells stained with isotype-matched control antibodies. M indicates marker. Results of 1 of 3 independent dendritic cell preparations are shown.