PAR1 activation stimulates expression of MCP-1 in human monocytes and monocyte-derived human macrophages. (A) Monocytes. MCP-1 mRNA expression. Cells incubated in the presence or absence of thrombin, PAR1-AP, WEDE15, or control IgG for 8 hours; mRNA was extracted and subjected to RT-PCR. GAPDH transcript levels were used for normalization. Results of 1 of 3 experiments are shown. (B) Monocytes. MCP-1 release. Cells were incubated in the presence or absence of the stimuli for 24 hours before MCP-1 release was analyzed by ELISA; results are the mean ± SEM of 6 independent experiments; 100% = 1.18 ± 0.34 ng/106 cells. (C) Macrophages. MCP-1 mRNA expression. Experimental conditions were the same as for monocytes. (D) Macrophages. MCP-1 release. Cells were treated as described for monocytes. Results are the mean ± SEM of 4 independent experiments. 100% = 5.2 ± 1.2 ng/106 cells. **P < .01 versus control (B, D).
