Comparison of LPS-mediated gene profiles between MCs and PBMNCs. Human MCs and PBMNCs were exposed to LPS for 6 hours. Each experiment contained one or 2 independent donors, and each component was repeated using different sets of donors. The data are representative of 2 separate analyses. (A) Overlapping sets of LPS-regulated genes expressed in human MCs and PBMNCs. Numbers in the overlapping region of the Venn diagram represent shared regulated genes. Numbers of MC- or PBMNC-specific genes are shown inside the stippled circles. Cell-specific genes were selected if the ratio of the relative expression level between stimuli was larger than 2.0, or if the data passed a stringent stimulus-specific filter based on the score. See the Blood website for supplemental data. (B) Representation of mRNA expression levels in control MCs, LPS-stimulated MCs, control PBMNCs, and LPS-stimulated PBMNCs. One row of colored bars represents one gene, and each column represents one stimulus. Color bars capture the magnitude of the response for each gene, according to the scale shown. Genes are placed in groups corresponding to pair-wise overlaps shown in the accompanying Venn diagrams. From top to bottom: LPS-stimulated MC-specific genes; and LPS-stimulated PBMNC-specific genes. A dendrogram shows overall similarity of the expression profiles of the representative samples.