Figure 5.
![Figure 5. Immunohistochemical localization of YS SP cells. (A) Dot plots: (i) 10.5 dpc YS Hoechst profile of all cells, (ii) cKit staining of 10.5 dpc YS (2.31% of all 10.5 dpc YS viable cells are cKitHI), and (iii) gating of cKitHI 10.5 dpc YS cells illustrate that they fall within the SP region. (B) cKit antibody staining of 10.5 dpc YS tissue sections finds cKitHI cells (arrowheads) in the mesodermal layer of the YS (cell nuclei DAPI [4,6 diamidino-2-phenylindole] stained; endoderm pseudocolored green). Original magnification, × 1000. (C) Whole mount staining of YS isolated 10.5 dpc for cKit demonstrates clusters of cells within and along the endothelium of large blood vessels.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/7/10.1182_blood-2003-01-0118/6/h81935014005.jpeg?Expires=1735550876&Signature=S5ShrKkF5gbBcdodFiMBEhC2FACk9VDAlFYDmwg~WWvMgZBG6XfVvhi1uPkxX9Ls1TyTeKSYbIa8lxdDDaXmhu5voLjC01WelqAsZqvO02DKZrvUkWezGjfRTASw7alae9FV7lumK6LlfDLUJ7rNRoH2SJyf9kgeY1Ab8QQAH5xZZDOdjfDBT~fm76ZgVYVbRHwDrRmNF79TU9E9Ncg2Hu1rZpB2kOCp9jCDFeZ6eOoIz75OPODGj6x2CMMQq-AjQESd1rvUx--qQTcvxzRiW1oRmyo5BKKshgv2J-67O8mdLw9fWf1BlVvAXpERCCLwI4BTnJsEJuN4lfQ3wkvUkw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Immunohistochemical localization of YS SP cells. (A) Dot plots: (i) 10.5 dpc YS Hoechst profile of all cells, (ii) cKit staining of 10.5 dpc YS (2.31% of all 10.5 dpc YS viable cells are cKitHI), and (iii) gating of cKitHI 10.5 dpc YS cells illustrate that they fall within the SP region. (B) cKit antibody staining of 10.5 dpc YS tissue sections finds cKitHI cells (arrowheads) in the mesodermal layer of the YS (cell nuclei DAPI [4,6 diamidino-2-phenylindole] stained; endoderm pseudocolored green). Original magnification, × 1000. (C) Whole mount staining of YS isolated 10.5 dpc for cKit demonstrates clusters of cells within and along the endothelium of large blood vessels.
