Comparison of induced Bax and endogenous Bax. (A) Comparison between PA-induced Bax and endogenous Bax in 293 cells. Following induction with 6 μ MPA, proteins were extracted at the indicated times and 100 μg lysate of each time point was subjected to SDS-PAGE and Western blotting with anti-Bax antibody (Cell Signaling Technology; reactive to both the induced mouse Bax and endogenous Bax of human 293 cell); (B) Comparison of endogenous Bax between different cell lines. 100 μg lysate from each cell line was subjected to Western blotting with the anti-Bax antibody described above.