Figure 4.
![Figure 4. p18 Bax has a reduced half-life that can be enhanced by ALLM. (A) The half-life of WT and p18 Bax was first determined by [35S]methionine metabolic labeling and pulse-chase as described in “Materials and methods.” (B) The half-life of WT, Asp33Ala, p18, and Glu6Ala Bax was determined again using the cyclohexamide method as described in “Materials and methods.” (C) The effect of ALLM on Bax protein half-life was determined by cyclohexamide method. Half-life was determined by densitometry analysis.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/7/10.1182_blood-2003-01-0211/6/h81935030004.jpeg?Expires=1735519052&Signature=A9rWVw0qn1NkQ7kj86X4KdcXV~upmIiM0h6XQ58ZyXBm1~ArBuO-TApsCN24ykTVcHFfNM9WEIhfOPD4VkUlqFogEAAPkNHg6Zhd0zFCWtRIAL2VTTf8WP5BAb1ojHwf2Z3RNnBTBXeMlkd0Kckl8cKgw3vQaZoaTN75dcFOQsmssQvd5X15EvLtfjY2ZxuAaF43sVNJKGCj5RxHxk4O-62pe-Sbn1vDyzOGcSb3PCBRXEJ-wEeMXn1XUqVWoeBzrZqbZAdha3s2NAaLXqZNiIlaVCoErdyW6mbNQJfW8AJyHZNCDuybOXHMXjKw1B2FJ9sbhnof2m-tUyC5urUenA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
p18 Bax has a reduced half-life that can be enhanced by ALLM. (A) The half-life of WT and p18 Bax was first determined by [35S]methionine metabolic labeling and pulse-chase as described in “Materials and methods.” (B) The half-life of WT, Asp33Ala, p18, and Glu6Ala Bax was determined again using the cyclohexamide method as described in “Materials and methods.” (C) The effect of ALLM on Bax protein half-life was determined by cyclohexamide method. Half-life was determined by densitometry analysis.
