Expression of Asp33Ala or p18 Bax induces increased caspase-9/-3 activation compared with WT or Glu6Ala Bax. Caspase-3 activity assay was performed following inducible Bax expression as described in “Materials and methods.” For caspase-9/-3 cleavage studies, proteins were extracted and subjected to SDS-PAGE and Western blotting with cleaved caspase-9/-3-specific antibodies and full-length procaspase-9/-3-specific antibodies, respectively. (A) Time course caspase-3 activity and caspase-9/-3 cleavage following inducible Bax expression. (B) Caspase-3 activity and caspase-9/-3 cleavage following inducible Bax expression in the presence of 40 μM ALLM. Error bars indicate means ± SD (n = 3).