Figure 6.
Figure 6. Expression of Asp33Ala or p18 Bax induces more cytochrome c release than WT or Glu6Ala Bax. Following inducible expression, cytosolic and mitochondrial fractions were generated using a digitonin-based subcellular fractionation technique. Equal amounts (50 μg) of mitochondrial and cytosolic fractions were subjected to SDS-PAGE and Western blotting with anticytochrome c antibody and, for negative control, antiprohibitin antibody. (A) Time course cytochrome c release following PA-inducible Bax expression. (B) Cytochrome c release at 24 hours following PA-inducible Bax expression in the absence or presence of 40 μM ALLM.

Expression of Asp33Ala or p18 Bax induces more cytochrome c release than WT or Glu6Ala Bax. Following inducible expression, cytosolic and mitochondrial fractions were generated using a digitonin-based subcellular fractionation technique. Equal amounts (50 μg) of mitochondrial and cytosolic fractions were subjected to SDS-PAGE and Western blotting with anticytochrome c antibody and, for negative control, antiprohibitin antibody. (A) Time course cytochrome c release following PA-inducible Bax expression. (B) Cytochrome c release at 24 hours following PA-inducible Bax expression in the absence or presence of 40 μM ALLM.

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