Impact of sustained ICN1 expression on T-cell development from CD34^lo thymic pro-T cells. Flow cytometry analysis of either CD3 versus TCRαβ or TCRγδ expression (A) or CD4 versus CD8 expression (B) performed after 30 or 19 days of FTOC, respectively, on electronically gated GFP⁺ cells generated from ICN1-transduced (ICN1) or mock-transduced (GFP) CD34^lo thymic pro-T cells. Results are representative of 3 independent experiments.