American Society of Hematology

Figure 1.

$Figure 1. Representative example of a biclonal B-CLPD case initially suspected because of the presence of 2 phenotypically different B-cell populations (CD19+/CD5+/FMC7– and CD19+/CD5–/FMC7+). (A-C) The reactivity of both B-cell subsets for FMC7 and CD5 on CD19+ gated cells before (A) and after (B-C) sorting of each B-cell subset. CD5–FMC7+ cells are shown in panel B, and CD5+FMC7– cells are shown in panel C. (D) Molecular amplification of VDJH rearrangements of the IgH genes in the whole PB samples (lane 1) and the purified fractions of CD5–/FMC7+/CD19+ (lane 2) and CD5+/FMC7–/CD19+ (lane 3) FACS-sorted B cells are displayed. As shown, the 2 sorted B-cell populations had different VDJH gene rearrangements. The numbers to the right of the blot indicate kilodaltons.$

Representative example of a biclonal B-CLPD case initially suspected because of the presence of 2 phenotypically different B-cell populations (CD19⁺/CD5⁺/FMC7^– and CD19⁺/CD5^–/FMC7⁺). (A-C) The reactivity of both B-cell subsets for FMC7 and CD5 on CD19⁺ gated cells before (A) and after (B-C) sorting of each B-cell subset. CD5^–FMC7⁺ cells are shown in panel B, and CD5⁺FMC7^– cells are shown in panel C. (D) Molecular amplification of VDJH rearrangements of the IgH genes in the whole PB samples (lane 1) and the purified fractions of CD5^–/FMC7⁺/CD19⁺ (lane 2) and CD5⁺/FMC7^–/CD19⁺ (lane 3) FACS-sorted B cells are displayed. As shown, the 2 sorted B-cell populations had different VDJH gene rearrangements. The numbers to the right of the blot indicate kilodaltons.

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